Segregation of Triazine-Resistant Chloroplasts after Introduction into Winter Type Oilseed Rape (Brassica napus L.) by Protoplast Fusion
نویسندگان
چکیده
Triazine-resistant chloroplasts o f the Canadian spring oilseed rape variety OAC Triton were transferred into four German winter oilseed rape lines and two cultivars o f double-low quality by means o f protoplast fusion. X-irradiation has been used to reduce the amount o f nuclear D N A o f the spring type cultivar and to promote cybrid formation. RFLP-analysis showed that some regenerants and their progeny carried both types o f chloroplasts. In some instances regenerants and progeny containing mixtures o f both chloroplasts not kept under selective conditions lost their triazine-resistant chloroplasts completely during further plant growth. Preliminary results o f greenhouse and field experiments indicate that volunteer plants can be eliminated by application o f 150-300 g/ha metribuzin (SencorR, Bayer AG) in a stand o f triazine-resistant oilseed rape o f double-low quality.
منابع مشابه
The Use of Herbicides in Biotech Oilseed Rape Cultivation and in Generation of Transgenic Homozygous Plants of Winter Oilseed Rape (Brassica napus L.)
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